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KMID : 0385020020180030104
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Korean Journal of Laboratory Animal Science
2002 Volume.18 No. 3 p.104 ~ p.119
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The Tissue Distribution and Overexpression of the Mouse Extracellular Superoxide Dismutase (EC-SOD) Gene in a Murine Cell Line, Pam212
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Lee, Sang-Rae
Oh, Seung-Hun/Nam, Yoon-Yi/Oh, Yang-Seok/Kim, Tae-Yoon/Suh, Jun-Gyo
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Abstract
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To evaluate the expression level of extracellular superoxide dismutase (EC-SOD) in various tissues, we purified EC-SOD from skin, lung, brain, kidney, liver and spleen of 4 and 8 weeks old FVB/NJ mice and measured its activity. EC-SOD activity in the skin was 10- to 19-fold higher than other tissues. This suggests that EC-SOD may be expressed massively in the skin and this enzyme may play an important role in the skin. Expressions of EC-SOD gene in vectors-transfected Pam212 cells were over two times higher than in control in vitro analysis. And EC-SOD activities in the cultured media of vectors-transfected cells were 5- to 6-fold higher than that of control. These results suggest that the vectors may not only induce keratinocyte-specific expression of EC-SOD but also express successfully functional EC-SOD.
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KEYWORD
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